Coding

Part:BBa_K091109:Design

Designed by: Andrew Gordon   Group: iGEM08_Davidson-Missouri_Western   (2008-06-19)


LuxS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 155
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 253
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nucleotide position 54 changed from A to T in order to destroy a PstI site; this is a sense mutation Also changed stop codon from TAG to TAA (recommended in Registry Help)



Source

The LuxS sequence was pulled from E. coli MG1655 by predesigned primers.

References